Chess2FLYA

Chemical shift statistics to FLYA

Chess2FLYA is a C++ program that uses RNA secondary structure information in the connectivity table format (.ct file format) and a built-in chemical shift statistics file to generate input files for the FLYA automated resonance assignment algorithm implemented in the program package CYANA. The input files

• CYANA sequence file (.seq) with the RNA sequence
• XEASY chemical shift list (.prot) with the mean values and standard deviations from 1D shift statistics statistics
• CYANA torsion angle restraint file (.aco) that restricts the backbone of regular A-form RNA
• CYANA macro file indicating the ranges of nucleotides involved in regular Watson-Crick base-pairs.

Availability

• Chess2FLYA is freely available from the group of Prof. F. H. Allain at ETH Zürich. The current version is Chess2FLYA 1.0.

Documentation

• README

References

• Aeschbacher, T., Schmidt, E., Blatter, M., Maris, C., Duss, O., Allain, F. H.-T., Güntert, P. & Schubert, M. Automated and assisted RNA resonance assignment using NMR chemical shift statistics. Nucl. Acids Res., submitted.