Solmaz Sobhanifar
PhD Candidate (on right)
Research Topic: NMR structural investigation of cell-free expressed membrane proteins involved in Alzheimer's Disease
Education
MSc. Dept Experimental Medicine, University of British Columbia (Canada)
BSc. Dept Biochemistry, University of British Columbia (Canada)
Contact: Sobhanifar@bpc.uni-frankfurt.de
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Publications
Reckel S, Sobhanifar S, Durst F, Löhr F, Shirokov VA, Dötsch V, Bernhard F. Strategies for the cell-free expression of membrane proteins. Methods in Molecular Biology (in press)
Sobhanifar S, Reckel S, Junge F, Schwarz D, Kai L, Karbyshev M, Löhr F, Bernhard F, Dötsch V. (2009) Cell-free expression and stable isotope labelling strategies for membrane proteins. J Biomol NMR (Epub ahead of print)
Reckel S*, Sobhanifar S*, Schneider B, Junge F, Schwarz D, Durst F, Löhr F, Güntert P, Bernhard F, Dötsch V. (2008) Transmembrane segment enhanced labeling as a tool for the backbone assignment of {alpha}-helical membrane proteins. Proc Natl Acad Sci USA 105:8262-7 (*equal contribution)
Schwarz D, Junge F, Durst F, Frölich N, Schneider B, Reckel S, Sobhanifar S, Dötsch V, Bernhard F. (2007) Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems. Nat Protoc 2:2945-57.
Klammt C, Schwarz D, Lehner I, Sobhanifar S, Löhr F, Zeelen J, Glaubitz C, Dötsch V, Bernhard F. (2007) Cell-free expression of integral membrane proteins for structural studies. In: Cell-free Protein synthesis, Methods and Protocols, A. Spirin / J. R. Swartz (eds.) Wiley-VCH, Weinheim, ISBN: 978-3-527-31649-6.
Sobhanifar S, Aquino-Parsons C, Stanbridge EJ, Olive P. (2005) Reduced expression of hypoxia-inducible factor-1alpha in perinecrotic regions of solid tumors. Cancer Res 65:7259-66.
Sobhanifar S, Long J. (2003) The yeast two hybrid assay: an excercise in experimental eloquence. Science creative quarterly; http://www.scq.ubc.ca/the-yeast-two-hybrid-assay-an-exercise-in-experimental-eloquence/
