Frank Bernhard

Group Leader

Institute of Biophysical Chemistry
Biocenter, Riedberg Campus
Goethe University
Max-von-Laue Str.9
60438 Frankfurt/Main

List of publications

Zocher, M., Roos, C., Wegmann, S., Bosshart, P.D., Dötsch, V., Bernhard, F., and Müller, D.J. (2011) Single-molecule force spectroscopy from nanodiscs: An assay to quantify folding, stability, and interactions on native membrane proteins. ACS Nano, DOI: 10.1021/nn204624p

Ma, Y., Münch, D., Schneider, T., Sahl, H.G., Bouhss, A., Ghoshdastider, U., Wang, J., Doetsch, V., Wang, X., and Bernhard, F. (2011) Preparative scale cell-free production and quality optimization of MraY homologues in different expression modes. J. Biol. Chem. 286, 38844-53

Reckel, S., Gottstein, D., Stehle, J., Löhr, F., Verhoefen, M.K., Takeda, M., Silvers, R., Kainosho, M., Glaubitz, C., Wachtveitl, J., Bernhard, F., Schwalbe, H., Güntert, P., and Dötsch, V. (2011) Solution structure of proteorhodopsin. Angewandte Chemie, DOI: 10.1002/anie.201105648.

Keller, T., Egenberger, B., Gorboulev, V., Bernhard, F., Uzelac, Z., Gorbunov, D., Witte, C., Koppatz, S., Doetsch, V., Hunte, C., Sitte, H.H., and Koepsell, H. (2011) The large extracellular loop of organic cation transporter 1 influences substrate affinity and is pivotal for oligomerization. J. Biol. Chem. 286, 37874-86

Matthies, D., Haberstock, S., Joos, F., Dötsch, V., Vonck, J., Bernhard, F., and Meier, T. (2011) Cell-free expression and assembly of a macromolecular membrane protein complex. J. Mol. Biol., 413, 593-603.

Rath, P., Demange, P., Saurel, O., Tropis, M., Daffé, M., Doetsch, V., Ghazi, A., Bernhard, F., and Milon, A. (2011) Functional expression of the PorAH channel from Corynebacterium glutamicum in cell-free expression systems: Implications for the role of the natural occuring mycolic acid modification. J. Biol. Chem. 286, 32525-32

Schmöe, K., Rogov, V.V., Rogova, N.Y., Löhr, F., Güntert, P., Bernhard, F., and Dötsch, V. (2011) Structural insights into Rcs phosphotransfer: The new identified RcsD-ABL domain enhances interaction with the response regulator RcsB. Structure 19, 577-87.

Rogov, V.V., Rogova, N.Y., Bernhard, F., Löhr, F., and Dötsch, V. (2011) A disulphide bridge network determines structure and function of the outer membrane protein RcsF. J. Biol. Chem., 286, 18775-83

Stefer, S., Reitz, S., Wang, F., Wild, K., Pang, Y.Y., Schwarz, D., Bomke, J., Hein, C., Löhr, F., Bernhard, F., Venic, V., Dötsch, V. and Sinnig, I. (2011) Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex. Science 333, 758-62.

Kai, L., Roos, C., Haberstock, S., Proverbio, D., Ma, Y., Junge, F., Karbyshev, M., Xu, Z., Dötsch, V., and Bernhard, F. (2011) Systems for the cell-free synthesis of proteins. Methods Mol. Biol., 800, 201-25.

Kai, L., Kaldenhoff, R., Lian, J., Zhu, X., Dötsch, V., Bernhard, F., Cen, P. & Xu, Z. Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes. PLoS One 2010 Sep 24;5(9):e12972

Junge F., Haberstock S., Roos C., Stefer S., Proverbio D., Dötsch V., Bernhard F. Advances in cell-free synthesis for the functional and structural analysis of membrane proteins. N Biotechnology epub ahead of print

Junge, F., Luh, L. M., Proverbio, D., Schäfer, B., Abele, R., Beyermann, M., Dötsch, V. & Bernhard, F. Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor.J. Struct. Biol. 172, 94-106 (2010)

Sobhanifar, S., Schneider, B., Löhr, F., Gottstein, D., Ikeya, T., Filipek, S., Güntert, P., Bernhard, F. & Dötsch, V. Structural investigation of the C-terminal catalytic fragment of presenilin-1 Proc. Natl. Acad. Sci. USA 107, 9644–9649 (2010)

Schwarz, D., Daley, D., Beckhaus, T., Dötsch, V. & Bernhard, F. Cell-free expression profiling of E. coli inner membrane proteins. Proteomics 10, 1762-1779 (2010)

Koeberle, A., Rossi, A., Zettl, H.,Pergola, C.,Dehm, F., Bauer, J., Greiner, C., Reckel, S.,Hoernig, C., Northoff, H., Bernhard, F., Dötsch, V.,Sautebin, L., Schubert-Zsilavecz, M. & Werz, O. The molecular pharmacology and in vivo activity of 2-(4-Chloro-6-(2,3-dimethylphenylamino)pyrimidin-2-ylthio)octanoic acid (YS121), a dual inhibitor of microsomal prostaglandin E-2 Synthase-1 and 5-Lipoxygenase. J. Pharmacol. Exp. Ther. 332, 840-848 (2010)

Sobhanifar, S., Reckel, S., Junge, F., Schwarz, D., Kai, L., Karbyshev, M., Löhr, F., Bernhard, F. & Dötsch, V. Cell-free expression and stable isotope labelling strategies for membrane proteins. J. Biomol. NMR 46, 33-43 (2010)

Koglin, A., Dötsch, V., and Bernhard, F. Molecular engineering aspects for the production of new and modified biosurfactants. Biosurfactants, Landes Bioscience, Austin.

Schneider, B., Junge, F., Shirokov, V.A., Durst, F., Schwarz, D., Dötsch, V.,& Bernhard, F. Membrane protein expression in cell-free systems. Methods Mol. Biol. 601, 165-186 (2010)

Reckel. S., Sobhanifar, S., Durst, F., Löhr, F., Shirokov, V. A., Dötsch, V. & Bernhard, F. Strategies for the cell-free expression of membrane proteins. Methods Mol. Biol. 607, 187-212 (2010)

Busche, A.E., Aranko, A.S., Talebzadeh-Farooji, M., Bernhard, F., Dötsch, V. & Iwai, H. (2009). Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein. Angew. Chem. Int. Ed. 48, 6128-6131 (2009)

Schwarz, D., Dötsch, V., and Bernhard, F. (2008) Production of membrane proteins using cell-free expression. Proteomics, 8, 3933-3946.

Koglin, A., Löhr, F., Bernhard, F., Rogov, V.R., Frueh, D.P., Strieter, E.R., Mofid, M.R., Güntert, P., Wagner, G., Walsh, C.T., Marahiel, M.A. and Dötsch, V. (2008) Structural basis for the selectivity of the external thioesterase of the surfactin synthetase. Nature, 454, 907-912.

Junge, F., Schneider, B., Reckel, S., Schwarz, D., Dötsch, V. and Bernhard, F. (2008) Large scale production of functional membrane proteins. Cell. Mol. Life Sci., 65, 1729-55.

Reckel, S., Sobhanifar, S., Schneider, B., Junge, F., Schwarz, D., Durst, F., Löhr, F., Güntert, P., Bernhard, F. and Dötsch, V. (2008) Transmembrane segment enhanced labeling as a tool for the backbone assignment of a-helical membrane proteins. Proc. Natl. Acad. Sci. USA, 105, 8262-7.

Keller, T., Schwarz, D., Bernhard, F., Dötsch, V., Hunte, C., Gorbulev, V. and Koepsell, H. (2008) Cell Free Expression and functional reconstitution of eukaryotic drug transporters. Biochemistry, 47, 4552-64.

Schwarz, D., Junge, F., Durst, F., Frölich, N., Schneider, B., Reckel, S., Sobhanifar, S., Dötsch, V., and Bernhard, F. (2007) Preparative scale expression of membrane proteins in E. coli based continuous exchange cell-free systems. Nat. Protocols, 2, 2945-57.

Schwarz, D., Klammt, C., Koglin, A., Durst, F., Löhr, F., Dötsch, V., and Bernhard, F. (2007) Cell-Free Expression Approaches for the Production and Characterization of Membrane Proteins. In: Cell-Free Protein Expression, pp 84-100, W.A. Kudlicki / F. Katzen / R.P. Bennett (eds.), Landes Bioscience, Austin.

Klammt, C., Srivastava, A., Eifler, N., Junge, F., Beyermann, M., Schwarz, D., Michel, H., Dötsch, V., and Bernhard, F. (2007). Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation. FEBS J., 274, 3257-69.

Klammt, C., Schwarz, D., Lehner, I., Sobhanifar, S., Löhr, F., Zeelen, J., Glaubitz, C., Dötsch, V., and Bernhard, F. (2007) Cell-free expression of integral membrane proteins for structural studies. In: Cell-free Protein synthesis, Methods and Protocols, A. Spirin / J. R. Swartz (eds.), Wiley-VCH, Weinheim, ISBN: 978-3-527-31649-6.

Klammt, C., Schwarz, D., Eifler, N., Engel, A., Piehler, J., Haase, W., Hahn, S., Dötsch, V., and Bernhard, F. (2007) Cell-free production of G-protein coupled receptors for functional and structural studies. J. Struct. Biol., 158, 482-93.

Bernhard, F., Klammt, C., and Rüterjans, H. (2007) Recombinant DNA and protein expression. In: Comprehensive Medicinal Chemistry II, Volume 3 – Drug discovery technologies, chapter 3.06 (Target search), pp. 107-129, H. Kubinyi (ed.), Elsevier, Amsterdam, Boston, Heidelberg.

Schwarz, D., Klammt, C., Koglin, A., Löhr, F., Schneider, B., Dötsch, V., and Bernhard F. (2007). Preparative scale cell-free expression systems: New tools for the large scale preparation of integral membrane proteins for functional and structural studies. Methods, 41, 355-69.

Rogov, V.V., Rogova, N., Bernhard, F., Koglin, A., Löhr, F., and Dötsch V. (2006). A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidin-kinase and phosphoreceiver domains. J Mol. Biol., 364, 68-79.

Klammt, C., Schwarz, D., Löhr, F., Schneider, B., Dötsch, V., and Bernhard, F. (2006). Cell-free expression as an emerging technique for the large scale production of integral membrane protein. FEBS J., 273, 4141-53.

Klammt, C., Schwarz, D., Dötsch, V., and Bernhard, F. (2006). Cell-free production of integral membrane proteins on a preparative scale. Meth. Mol. Biol., 375, 57-78.

Koglin, A., Mofid, M.R., Löhr, F., Schäfer, B., Rogov, V.V., Blum, M.M., Mittag, T., Marahiel, M., Bernhard, F., and Dötsch, V. (2006). Dual Two-state conformational equilibrium as molecular basis for the function of integrated peptidyl carrier proteins in nonribosomal peptide synthetase systems. Science, 312, 273-276.

Koglin, A., Klammt, C., Trbovic, N., Schwarz, D., Schneider, B., Schäfer, B., Löhr, F., Bernhard, F., Dötsch, V. (2006). Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins. Magn. Reson., 44, 17-23.

Klammt, C., Schwarz, D., Fendler, K., Haase, W., Dötsch, V., and Bernhard, F. (2005). Evaluation of detergents for the soluble expression of -helical and ß-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J., 272, 6024-38.

Löhr, F., Rogov, V.V., Shi, M., Bernhard, F., and Dötsch, V. (2005). Triple-Resonance Methods for Complete Resonance Assignment of Aromatic Protons and Directly Bound Heteronuclei in Histidine and Tryptophan Residues. J. Biomol. NMR, 32, 309-28.

Trbovic, N., Klammt, C., Koglin, A., Löhr, F., Bernhard, F., and Dötsch, V. (2005). Efficient strategy for the rapid backbone assignment of membrane proteins. J. Am. Chem. Soc., 127, 13504-5.

Rogov, V.V., Bernhard, F., Löhr, F., and Dötsch, V. (2004) Solution Structure of the Escherichia coli YojN Histidine-Phosphotransferase Domain and its Interaction with Cognate Phosphoryl Receiver Domains. J. Mol. Biol., 343, 1035-48.

Rogov, V.V., Löhr, F., Bernhard, F., and Dötsch, V. (2004) Letter to the Editor: Assignment of 1H, 13C and 15N resonances of the Escherichia coli YojN Histidine-Phosphotransferase (HPt) domain. J. Biomol. NMR, 30, 103-4.

Steller, S., Sokoll, A., Wilde, C., Bernhard, F., Franke, P., and Vater, J. (2004) The initiation of surfactin biosynthesis and the role of the SrfD-thioesterase protein. Biochemistry, 43, 11331-43.

Klammt, C., Löhr, F., Schäfer, B., Haase, W., Dötsch, V., Rüterjans, H., Glaubitz, C., and Bernhard, F. (2004) High level cell-free expression and specific labeling of integral membrane proteins. Eur. J. Biochem., 271, 568-80.

Klammt C., Bernhard F., and Rüterjans H. (2004) 13C- and 15N-isotopic labeling of proteins. In: Molecular Biology in Medical Chemistry, T. Dingermann, G. Folkers, H. Steinhilber (eds.), pp. 269-299, Wiley-VCH, Weinheim.

Sengupta K., Klammt C., Bernhard F., and Rüterjans H. (2003) Incorporation of fluorescence labels into cell-free produced proteins. In: Cell-Free Protein Expression, J.R. Swartz (ed.) pp. 81-88, Springer Verlag, Berlin, Heidelberg, New York.

Pristovsek P., Sengupta K., Löhr F., Schäfer B., von Trebra M.W., Rüterjans H., and Bernhard F. (2003) Structural analysis of the DNA-binding domain of the Erwinia amylovora RcsB protein and its interaction with the RcsAB box. J. Biol. Chem., 278, 17752-9.

Symmank H., Franke P., Saenger W., and Bernhard F. (2002) Modification of biologically active peptides: production of a novel lipohexapeptide after engineering of Bacillus subtilis surfactin synthetase. Protein Eng., 15, 913-21.

Minogue T.D., Wehland von Trebra M., Bernhard F., and von Bodman S.B. (2002) The autoregulatory role of EsaR, a quorum-sensing regulator in Pantoea stewartii ssp. stewartii: evidence for a repressor function. Mol. Microbiol., 44, 1625-35.

Wehland M., and Bernhard F. (2000) The RcsAB-box: Characterization of a new operator essential for the regulation of EPS biosynthesis in enteric bacteria. J. Biol. Chem. 275, 7013-7020.

Duitman E.H., Hamoen L.W., Rembold M., Venema G., Seitz H., Saenger W., Bernhard F., Reinhardt R., Schmidt M., Ulrich C., Stein T., Leenders F., and Vater J. (1999) The mycosubtilin synthetase of Bacillus subtilis ATCC 6633: A multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase. Proc. Natl. Acad. Sci. U.S.A. 96, 13294-13299.

Wehland M., Kiecker C., Coplin D.L., Kelm O., Saenger W., and Bernhard F (1999). Identification of an RcsA/RcsB recognition motif in the promoters of exopolysaccharide biosynthetic operons from Erwinia amylovora and Pantoea stewartii subspecies stewartii. J. Biol. Chem. 274, 3300-3307.

Symmank H., Saenger W., and Bernhard F. (1999). Analysis of engineered multifunctional peptide synthetases: Enzymatic evaluation of surfactin synthetase domains in hybrid bimodular systems. J. Biol. Chem. 274, 21581-21588.

Aldridge P., Bernhard F., Bugert P., Coplin D.L., and Geider K. (1998). Characterization of a gene locus from Erwinia amylovora with regulatory functions in exopolysaccharide synthesis of Erwinia spp. Can. J. Microbiol. 44, 657-666.

Elsner A., Engert H., Saenger W., Hamoen L., Venema G., and Bernhard F. (1997). Substrate specificity of hybrid modules from peptide synthetases. J. Biol. Chem. 272, 4814-4819.

Kelm O., Kiecker C., Geider K., and Bernhard F. (1997). Interaction of the regulator proteins RcsA and RcsB with the promoter of the operon for amylovoran biosynthesis in Erwinia amylovora. Mol. Gen. Genet. 256, 72-83.

Bernhard F., Schullerus D., Bellemann P., Nimtz M., Coplin D.L., and Geider K. (1996). Genetic transfer of amylovoran and stewartan synthesis between Erwinia amylovora and Erwinia stewartii. Microbiology 142, 1087-1096.

Bernhard F., Demel G., Soltani K., v. Döhren H., and Blinov V. (1996). Identification of genes encoding for peptide synthetases in the gram-negative bacterium Lysobacter sp. ATCC 53042 and the fungus Cylindrotrichum oligospermum. DNA Sequence 6, 319-330.

Coplin D. L., Bernhard F., Majerczak D., and Geider K. (1994). Capsular polysaccharide synthesis in Erwinias, p. 341-356. In C. L. Kado and J. H. Crosa (ed.). Molecular mechanisms of bacterial virulence. Kluwer Academic Publishers, Dordrecht, The Netherlands.

Bernhard F., Coplin D.L., and Geider K. (1993). A gene cluster involved in amylovoran synthesis of Erwinia amylovora: Characterization and its relationship to cps genes in Erwinia stewartii. Mol. Gen. Genet. 239, 158-168.

Bernhard F., Bellemann P., Bugert P., Coplin D.L., and Geider K. (1993). Heterologous expression of EPS-genes in Erwinia amylovora and Erwinia stewartii. Acta Horticulturae 338, 265-272.

Schwartz T., Bernhard F., Theiler R., and Geider K. (1991). Diversity of the fireblight pathogen in production of dihydrophenylalanine, a virulence factor of some Erwinia amylovora strains. Phytopathology 81, 873-878.

Bernhard F., Poetter K., Geider K., and Coplin D. (1990). The rcsA Gene from Erwinia amylovora: Identification, Nucleotide Sequence and Regulation of Exopolysaccharide Biosynthesis. Mol. Plant Microbe Interact. 3, 429-437.

Geider K., Falkenstein H., Bellemann P., Jahn N., Schwartz T., Theiler R., and Bernhard F. (1990). Virulence factors of Erwinia amylovora. Acta Horticulturae 273, 227-231.